Composite

Part:BBa_K1638038:Design

Designed by: Jens Sivkr Pettersen   Group: iGEM15_SDU-Denmark   (2015-09-14)


Yhbj fused to T25 domain of CyaA with cAMP-induced RFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1851
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 712
    Illegal AgeI site found at 824
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragment of this part containing Plac-T25-yhbJ was synthesized by IDT as a gBlock gene fragment. Standard BioBrick assembly was used to construct the whole device including the mRFP generator controlled by PcstA (BBa_K861173).

Source

pKT25
yhbJ: Gene ID: 947727

References

[1]: Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.